Researchers make soybean cyst nematode test faster, easier

Researchers make soybean cyst nematode test faster, easier

New system to test for soybean cyst nematode is 20 times faster than previous methods

A group of University of Illinois researchers have developed a test for soybean cyst nematode that's approximately 20 times faster, and provides more precise data, than current methods.

According to Kaustubh Bhalerao, principal investigator for the project, producers typically lose 5% to 10% of their yield to SCN.

Related: You can test anytime for soybean cyst nematode

Identification and protection methods are required each new crop season. He says producers have to ask themselves questions such as: Did the resistant variety they used last year work? Should they use a different variety this year, or should they rotate to corn?

Looking for cysts: Here are soybean roots infected with cyst nematode nodules. (Photo courtesy of John Obermeyer, Purdue University Extension entomologist)

Those questions are often answered by soil sampling that can cost about $5 acre to get one result of "very poor reproducibility, quality, and spatial resolution," Bhalerao said.

The new system can bring that cost down markedly, he said; producers can get 20 points per 10 acres, or about two points per acre, which increases the spatial resolution by 20 times. Experts recommend 10 points per acre for the most accurate results, and Bhalerao said with properly streamlined operations, the results can be provided for about $20, or $2 per sample.

Related: Soybean Cyst Nematodes Cannot Be Ignored

The system includes an "extractor" that processes the soil samples to separate the eggs. One extractor unit has four channels for processing, so multiple units can be used as needed. An automated image acquisition and analysis system counts the eggs.

The extractor makes a "milkshake" out of a clod of dirt and water, Bhalerao explains. "The operator inserts a plug of soil, and water is added so that the cysts float and the dirt sinks. Once the cysts float, we raise the water level so the cysts flow out onto a set of sieves, where they're crushed by an automated roller. The eggs from the cysts pass through the sieves and are washed into a sample container. The eggs are stained and counted using the image analysis system."


A website will be developed so producers can monitor and organize the data they receive from the SCN testing and possibly integrate it with other information they collect, such as data from yield monitors on tractors, the University said.

Bhalerao said the research team hopes to provide this group of technologies primarily through soil testing companies.

Related: Soybean Cyst Nematodes Could Show Up in Problem Areas

"With the current technology, an average company is testing about 3,000 samples a year," he said. "With our technology, they should be able to do 30,000 or more."

Bhalerao is the head of a multidisciplinary research team that includes Farhan Syed Abbas, a graduate student in technical systems management in Agricultural and Biological Engineering; Allante Whitmore, a graduate student in ABE; Sadia Bekal, a research associate in Bhalerao's lab; Chinmay Soman, a former postdoctoral researcher in the lab; and Kris Lambert, an associate professor in crop sciences.

"My contribution includes the image analysis program," said Bhalerao. "Farhan did the grant submission and presentation, Allante has done all the engineering drawing for the extractor, and Kris is an advisor on the grant we received for the research.

"The agricultural industry is showing a lot of interest in this area," Bhalerao concluded. "It's kind of the 'wild west' right now, but we're positioned very well, and we have a good prototype in the market."

Source: University of Illinois

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